Virology

Virology

Clinical Virology Laboratory at the Institute provides a full range of diagnostic services for the detection and monitoring of viral illnesses in both the adult and pediatric patient. Most of the functioning of the department has been automated to an extent, in keeping with international standards. The laboratory performs diagnostic services for the detection and monitoring of viral diseases for the full range of Hepatitis viruses (Hepatitis A, B, C, D and E). The laboratory is fully equipped to diagnose and monitor all most all the viruses causing infections in transplant recipients and other immune compromised patients. A BSL level grade 2/3 molecular diagnostic facility is available in the molecular set up. Viral genotyping is routinely being done. Multiplex Real time PCRs is the strength of the laboratory including Respiratory viral PCR for 33 different pathogens together. The laboratory takes pride in performing several diagnostic assays for the first time in the country, like testing for drug resistant substitutions in HCV (RAS) , antiviral drug resistance testing in HBV; HBs Ag quantification in clinical samples ; testing for Occult HBV infection and ccc DNA in biopsy samples; screening for various mutations in HBV like Pre-core, Basal core promoter mutations; HEV RNA Quantification and genotyping; EBV and CMV Quantification in tissue biopsy samples. Furthermore, the laboratory is actively involved in the investigation of any outbreak that grips the city like recent Influenza, Dengue and Chikungunya outbreaks in the capital. Recently, point of care of Molecular diagnostics was started in the laboratory in assays like HCV RNA, H1NI and HIV viral load testing. Microarray platform for syndromic viral infection testing is also available in the laboratory. Virology laboratory takes pride in being made as one of the testing laboratories by Central Drugs Standard Control Organization for the validation of Hepatitis diagnostic kits. Virology laboratory at ILBS is also nominated by ICMR as one of the Apex Viral Hepatitis laboratories in the country to look after external quality assurance (EQAS) in various Virology labs across the Nation. The lab has been active in initiating teaching modules and programs in clinical virology. NABL accreditation was awarded to the lab in 2013 and is the one of the very few NABL accredited molecular virology lab in India. The Molecular Lab participates in EQAS by Royal College of Pathology, Australia. The Virology Lab has made an exhaustive protocol for Needle Stick Injuries, post exposure prophylaxis for healthcare professionals at ILBS and also keep a track of the HBV immunization status of the entire ILBS staff. The laboratory also supervises the unique Needle stick injury control programme through our website: nsi.ilbs.in. The ILBS NSI – website aims to provide platform for a centralized registry for needle stick injuries. The vision of ILBS is not only to create an institutional database on needle stick/ sharps injuries but to expand it as a statewide and thereafter national NSI Registry. A unique PDCC course in Hepatovirology has been introduced from August 2014 to train young budding microbiologists in this field. As a part of World Health Organization collaborative Center (WHO-CC) in Viral Hepatitis, Virology laboratory is actively involved in imparting capacity building training to Microbiologists and other laboratory staff across the Nation.

Services Rendered

Serology
  • Hepatotropic viruses(HBV, HCV, HAV,HEV and HDV)
  • Transplant related Herpes group (HCMV, EBV, HSV 1&2 and VZV)
  • Human Immunodeficiency Viruses 1&2 (HIV 1&2)
  • Dengue virus
  • Vector borne viruses: Dengue, Chickungunya. 
  • Special serological(quantification) assays – HBsAg quantification
Molecular Virology
  • Hepatotropic viruses(HBV, HCV, HAV and HEV) by real-time PCR
  • HBV and HCV genotyping and HBV mutation screen (Precore and Basal Core Promotor mutations).
  • Transplant related viruses (HCMV, EBV, HSV 1&2 ,VZV,BK)
  • HBV drug resistance testing by sequencing 
  • Occult HBV testing by sequencing
  • Real time PCR for HBV - ccc DNA in clinical samples.
  • Real time PCR for dengue typing.
  • Microarray based testing for Viral syndromes.
Needle Stick injury protocol
  • Post-exposure testing and counseling.
HBV vaccination and anti-HBs (titer determination)
  • HBV vaccination at recruitment
  • Determination of anti-HBs (titers) post vaccination
NewServices and Facilities started in Lab:
  • Pyrosequencing facility for various sequencing-based assays done in the lab like genotyping, drug resistance testing.
  • HCV core antigen test.
  • HDV RNA Quantitative (Real Time PCR) test.
Outreach:
  • Organized Hepatitis B & C screening camp on the occasion of Hepatitis Day on 4th Dec, 2018. A total of 207 registrations were done in the camp.
  • Organized Hepatitis B & C screening camp under project 'EMPATHY' at DMRC Bhawan Day on 30th Nov 2018. A total of 405 registrations were done in the camp.
  • As a part of the"Healthy liver and Healthy Delhi" project approximately 10,000 persons in Delhi have been screened for HBV and HCV infections..
 

Services Rendered

Investigations 2017 2018
HBV 21154 21722
HCV 10221 10335
HEV & HAV 3831 4259
Transplant Related Markers (HCMV, EBV, HSV 1&2 and VZV) 2328 2349
HIV 4125 4017
Dengue Virus 413 552
Chikungunya Igm 22 08
Special Serology (HBsAg Quantification) 1910 1638
ANTI- EBV IGM, ANTI-HSV IGM, ANTI- VZV IGM 2498 2328
HDV IgM, HDV Total 61 44
HBV DNA Quantitative (real Time Pcr) 2880 2966
HCV RNA Quantitative (real Time Pcr) 2254 2097
HAV & HEV RNA Quantitative (real Time Pcr) 144 103
Transplant Related Viruses Dna Quantitative (HCMV, EBV, HSV 1&2 and VZV) 803 969
Genotyping HBV & HCV 397 272
Hbv Drug Resistance Testing 08 06
Respiratory Virus PCR 139 190
HCV RAV Testing 13 88
Bk Virus (real Time Pcr) 34 17
Pre-core & Bcp Mutations Analysis 01 00
HIV-1 Viral load 01 10
H1N1(Swine Flu) PCR 110 167
Rapid HCV RNA Quantitative 18 45
Adeno CMV EBV PCR 01 01
HIV Viral load 06 05
Staff anti - HBs (titer) 323 -
HBsAg 148 130
Anti-HBs(titer) 148 130
Anti HCV 148 130
HBsAg 237 11202
Anti-HCV 237 11202
Total 52308 74867
 

New Services & Facilities

  • HIV 1 Viral load testing
  • Rapid HCV RNA Quantitative PCR by GeneXpert
  • H1N1 Qualitative PCR
  • Adeno CMV EBV multiplex PCR 

Courses Offered

Name of the course Number of Candidates
admitted (Year 2018)
Number of Candidates
Passed Out (Year 2018)
PDCC in Hepato - Virology Dr. Krithiga R, Dr. Neha A Sharma Dr. Dhara Shah Dr. Nitin Kumbhar Dr. Mohd. Saquib Dr. Krithiga R Dr. Neha A Sharma

Short Term Training

Name of the course Details of Candidates
Short Term Training in Clinical Virology Nimisha Upadhyay, DIPSAR, University

Visiting faculty

Name Institution/strong>
Dr. Nidhi Prasad IGIMS, Patna
Dr Anita Chakravarti MAMC, Delhi
Dr Mini P Singh PGI, Chandigarh

Educational Activities

Department of Clinical Virology conducted PG assembly on "Clinical Virology- Made Easy" under the aegis of IAMM-DC on 16th -17th August 2018. The program provided theoretical and practical update in the field of clinical virology with emphasis on Hepatitis Viruses and Transplant viruses. A total of 43 delegates from all over India actively participated

As a part of capacity building and laboratory network strengthening, Virology laboratory imparts regularly training to various levels of laboratory professionals on Viral Hepatitis testing. The Department organizes regularly one day hands-on technician training programme. This involves series of lectures on various aspects of Viral Hepatitis testing, hands on training in various laboratory techniques and group discussions. Till now more than 80 laboratory technicians from Delhi state AIDS control society have been trained by the Department

Organized a lunch symposium on “Approach to Syndromic testing in viral infections” on 24th August.

First operational meeting of "HCV Delhi demonstration project" was conducted on 14th Dec and various nodal officers from 5 hospitals in Delhi met and the Unitaid-supported Hepatitis C Elimination through Access to Diagnostics (HEAD-Start) project to enhance availability and accessibility of hepatitis C tests was initiated.

Representation of the Department by the Residents at various National Conferences:-

Shefali Gupta, Supriya Mahajan, Viniyendra Pamecha, Ekta Gupta. "Pre-transplant seroprevalence and post-transplant reactivation of opportunistic viral infections in solid organ transplant recipients: a single tertiary care center experience," awarded as the best poster award at 29th Annual Conference on Indian Society of Organ Transplantation October, 2018.

Krithiga Ramachandran, Reshu Agarwal, Chhagan Bihari,Vikram Bhatia , Ekta Gupta, "Role of Epstein Barr Virus (EBV) DNA PCR in diagnosing unresolved pyrexia of unknown origin with liver involvement" in MICRO - D - CON November 2018 at Rajiv Gandhi Super Speciality Hospital, New Delhi .

Nitin Kumbhar, Reshu Agarwal, Ashok Choudhary, Viniyendra Pamecha, Ekta Gupta "Hepatitis B core antibody positive liver grafts can be safely given in core antibody positive and negative recipients with thorough virological evaluation and monitoring" presented in INTERVIROCON 2018 ,12th -14th November 2018 at PGIMER, Chandigarh.

Krithiga Ramachandran, Manish Choudhary, Reshu Agarwal, Rakhi Maiwall, Bansidhar Tarai, Ekta Gupta "Emergence of a new subclade with endemic evolution in Haemaggulutinin(HA) gene among circulating Influenza A (H1N1)pdm09 strains in Delhi, oral presentation at 42nd Annual Conference of Indian Association of Medical Microbiologists organized by NIMHANS, Bangalore from 28th November- 2nd December 2018.

 

Faculty

Copyright © Institute of Liver & Biliary Sciences 2019. All rights reserved. Designed & Developed by Cyfuture