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Clinical Virology Laboratory at the Institute provides a full range of diagnostic services for the detection and monitoring of viral illnesses in both the adult and pediatric patient populations. The laboratory handles an enormous load and variety of hepatitis viral markers testing. This area of the department has been automated to the extent possible, in keeping with international standards. The laboratory performs diagnostic services for the detection and monitoring of viral diseases for the full range of Hepatitis viruses (Hepatitis A, B, C, D and E). Quantitative assessment of viral infection in transplant and other immunocompromised patients (CMV, EBV, and BK) is also done.
A BSL level grade 2/3 molecular diagnostic facility for HBV, HCV, HEV, HAV, CMV, EBV, HSV 1 & 2 and VZV is available. HBV & HCV genotyping are routinely being done. Our laboratory takes pride in performing the following diagnostic assays for the First time in India: Antiviral drug resistance testing for HBV; HBs Ag quantification; Occult HBV diagnosis; Mutant screen HBV (Pre-core, Basal core promoter mutations);HEV RNA Quantification; EBV Quantification; CMV and EBV Quantification from tissue biopsy.Furthermore, the lab is actively involved in the investigation of various viral outbreaks (Influenza and Dengue) in the community. Emphasis is given to quality research and development of novel diagnostics to remain at the forefront of patient care.
The Virology Lab has been active in initiating teaching modules and programs in clinical virology. NABL accreditation was awarded to the lab in 2013 and this is the one of the very few NABL accredited molecular virology lab in India. The Molecular Lab participates in External Quality Assurance Program (EQAS) by Royal College of Pathology, Australia. The Virology Lab has made an exhaustive protocol for Needle Stick Injuries for healthcare professionals at ILBS. The lab also keeps a record of immunization protocol of the entire ILBS staff.
A unique PDCC course in Hepatovirology has been introduced from August 2014 to train young budding microbiologists in this field. We provide opportunity for M.Sc. students from various institutions to observe virological techniques as a part of internship and Observership programmes. We expect to start drug resistance testing in HCV for the first time in the country. Virology laboratory is also actively involved in initiation of nationwide training program for Microbiologist and technical staff as a part of World Health Organization collaborative Center (WHO CC) in Viral Hepatitis.
Hepatotropic viruses(HBV, HCV, HAV,HEV and HDV)
Transplant related Herpes group (HCMV, EBV, HSV 1&2 and VZV)
Human Immunodeficiency Viruses 1&2 (HIV 1&2)
Liver SOL serological workup(Amoebic liver abscess, Hydatid cyst)
Special serological(quantification) assays – HBsAg quantification
Hepatotropic viruses(HBV, HCV, HAV and HEV) by real-time PCR
HBV and HCV genotyping and HBV mutation screen (Precore and Basal Core Promotor mutations).
Transplant related viruses (HCMV, EBV, HSV 1&2 ,VZV,BK)
HBV drug resistance testing by sequencing
Occult HBV testing by sequencing
Real time PCR for HBV - ccc DNA in clinical samples.
Real time PCR for dengue typing.
Needle Stick injury protocol
Post-exposure testing and counseling.
HBV vaccination and anti-HBs (titer determination)
HBV vaccination at recruitment
Determination of anti-HBs (titers) post vaccination
Integrated Counseling And Testing Centre (ICTC) Cervices For HIV.
NEEDLE STICK INJURY WEBSITE - AN ILBS INITIATIVE:
Department of Virology embarked on a mission to provide a support platform for health care workers outside the Institute as well, who have professional exposure to blood borne infections but do not have the requisite knowledge to manage them. ILBS has a website on Needle stick injuries which provides information, tools, and strategies to reduce workers' exposure to blood and body fluids of patients infected with blood borne pathogens, such as HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV). The ILBS NSI – website aims to provide platform for a centralized registry for needle stick injuries. The vision of ILBS is not only to create an institutional database on needle stick/sharps injuries but to expand it as a statewide and thereafter national NSI Registry.
HEV & HAV
Liver SOL (Amoebic & Hydatid Cyst)
Anti- EBV IgM, Anti-HSV IgM, Anti- VZV IgM
HBV DNA (Real Time quantitative)
HCV RNA (Real Time quantitative)
HAV & HEV RNA (Real Time quantitative)
Transplant related viruses DNA quantitative
Genotyping HBV & HCV
HBV drug resistance testing
DETERMINATION OF IMMUNITY TO HBV POST VACCINATION
Pre Employment Health Checkups
Tests done in Free Health camps-2015
New Services & Facilities
Real time PCR for cccDNA in clinical samples.
HBV mutant screen (Precore and Basal Core Promotor mutations).
Real time PCR for dengue typing.
Multiplex Real time PCR for Influenza Viruses(Influenza A, B and swine Flu).
Organized Viral Hepatitis screening camp on the occasion of World Hepatitis Day on 28th July, 2015. Total 187 registrations were done for the camp.
Organized Hepatitis B & C screening camp on the occasion of Hepatitis Day on 4th Dec, 2015. More than 200 registrations were done for the camp.
Representation of the Department by the residents at various National Conferences:
- "Hepatitis B vaccination status and anti-HBs antibody levels in health care workers at a tertiary care institute in north India: a six year survey" Pragya Ranjan, Neha Ballani, Amit Chaturvedi, Ekta Gupta, CIDSCON, 21-23 August, New Delhi.
- "A comparative analysis of three platforms for hepatitis C viral load testing" Pragya Ranjan, Ekta Gupta, Priyanka Menon, Guresh Kumar Chibbe, Shiv Kumar Sarin, APASL STC, 18-20 December, New Delhi.
- "Progression of Liver fibrosis in HCV genotype 3a is associated with modulation in let-7 miRNA family" Manish Choudhary, Sadaf Dar, Ekta Gupta, SyedvKazim, Gayatri Ramakrishna, Shiv Kumar Sarin APASL STC, 18-20 December, New Delhi.