Clinical Virology Laboratory at the Institute provides a full range of diagnostic services for the detection and monitoring of viral illnesses in both the adult and pediatric patient. Most of the functioning of the department has been automated to an extent, in keeping with international standards. The laboratory performs diagnostic services for the detection and monitoring of viral diseases for the full range of Hepatitis viruses (Hepatitis A, B, C, D and E). The laboratory is fully equipped to diagnose and monitor all most all the viruses causing infections in transplant recipients and other immune compromised patients. A BSL level grade 2/3 molecular diagnostic facility is available in the molecular set up. Viral genotyping is routinely being done. Multiplex Real time PCRs is the strength of the laboratory including Respiratory viral PCR for 33 different pathogens together. The laboratory takes pride in performing several diagnostic assays for the first time in the country, like testing for drug resistant substitutions in HCV (RAS) , antiviral drug resistance testing in HBV; HBs Ag quantification in clinical samples ; testing for Occult HBV infection and ccc DNA in biopsy samples; screening for various mutations in HBV like Pre-core, Basal core promoter mutations; HEV RNA Quantification and genotyping; EBV and CMV Quantification in tissue biopsy samples. Furthermore, the laboratory is actively involved in the investigation of any outbreak that grips the city like recent Influenza, Dengue and Chikungunya outbreaks in the capital. Recently, point of care of Molecular diagnostics was started in the laboratory in assays like HCV RNA, H1NI and HIV viral load testing. Microarray platform for syndromic viral infection testing is also available in the laboratory. Virology laboratory takes pride in being made as one of the testing laboratories by Central Drugs Standard Control Organization for the validation of Hepatitis diagnostic kits. Virology laboratory at ILBS is also nominated by ICMR as one of the Apex Viral Hepatitis laboratories in the country to look after external quality assurance (EQAS) in various Virology labs across the Nation. The lab has been active in initiating teaching modules and programs in clinical virology. NABL accreditation was awarded to the lab in 2013 and is the one of the very few NABL accredited molecular virology lab in India. The Molecular Lab participates in EQAS by Royal College of Pathology, Australia. The Virology Lab has made an exhaustive protocol for Needle Stick Injuries, post exposure prophylaxis for healthcare professionals at ILBS and also keep a track of the HBV immunization status of the entire ILBS staff. The laboratory also supervises the unique Needle stick injury control programme through our website: nsi.ilbs.in. The ILBS NSI – website aims to provide platform for a centralized registry for needle stick injuries. The vision of ILBS is not only to create an institutional database on needle stick/ sharps injuries but to expand it as a statewide and thereafter national NSI Registry. A unique PDCC course in Hepatovirology has been introduced from August 2014 to train young budding microbiologists in this field. As a part of World Health Organization collaborative Center (WHO-CC) in Viral Hepatitis, Virology laboratory is actively involved in imparting capacity building training to Microbiologists and other laboratory staff across the Nation.
- Hepatotropic viruses(HBV, HCV, HAV,HEV and HDV)
- Transplant related Herpes group (HCMV, EBV, HSV 1&2 and VZV)
- Human Immunodeficiency Viruses 1&2 (HIV 1&2)
- Dengue virus
- Vector borne viruses: Dengue, Chickungunya.
- Special serological(quantification) assays – HBsAg quantification
- Hepatotropic viruses(HBV, HCV, HAV and HEV) by real-time PCR
- HBV and HCV genotyping and HBV mutation screen (Precore and Basal Core Promotor mutations).
- Transplant related viruses (HCMV, EBV, HSV 1&2 ,VZV,BK)
- HBV drug resistance testing by sequencing
- Occult HBV testing by sequencing
- Real time PCR for HBV - ccc DNA in clinical samples.
- Real time PCR for dengue typing.
- Microarray based testing for Viral syndromes.
Needle Stick injury protocol
- Post-exposure testing and counseling.
HBV vaccination and anti-HBs (titer determination)
- HBV vaccination at recruitment
- Determination of anti-HBs (titers) post vaccination
|HEV & HAV||3391||3831|
|Special Serology (HBsAg Quantication)||1900||1910|
|EBV , HSV , VZV||180||193|
|HBV DNA Quantitative (Real Time PCR)||2956||2880|
|HCV RNA Quantitative (Real Time PCR)||3192||2254|
|HAV and HEV RNA Quantitative (Real Time PCR)||141||144|
|Transplant Related Viruses DNA Quantitative (CMV, EBV, HSV
1and2 and VZV)
|Genotyping HBV & HCV||500||397|
|HBV drug resistance testing||15||08|
|RESPIRATORY VIRUS PCR||40||139|
|HCV RAV TESTING||05||13|
|BK VIRUS (REAL TIME PCR)||18||34|
|PRE CORE & BCP MUTATIONS ANALYSIS||01||01|
|HIV Viral load||-||01|
|H1N1(Swine Flu) Qualitative||-||110|
|Rapid HCV RNA Quantitative||-||18|
|Adeno CMV EBV PCR||06|
|DETERMINATION OF IMMUNITY TO HBV POST VACCINATION|
|Pre Employment Health Checkups|
|Tests done in Free Health camps-2017|
New Services & Facilities
- HIV 1 Viral load testing
- Rapid HCV RNA Quantitative PCR by GeneXpert
- H1N1 Qualitative PCR
- Adeno CMV EBV multiplex PCR
As a part of capacity building and laboratory network strengthening, Virology laboratory imparts regularly training to various levels of laboratory professionals on Viral Hepatitis testing. The Department organizes regularly one day hands-on technician training programme. This involves series of lectures on various aspects of Viral Hepatitis testing, hands on training in various laboratory techniques and group discussions. Till now more than 80 laboratory technicians from Delhi state AIDS control society have been trained by the Department
Organized a lunch symposium on “Approach to Syndromic testing in viral infections” on 24th August.
Representation of the Department by the Residents at various National Conferences:-
Mousumi Paul, Oral presentation:- Secured third position in oral presentation in the topic “Increasing prevalence of Pre-core and Basal core promoter mutations seen in hepatitis B e antigen positive patients with chronic hepatitis B infection” in the National Virology conference conducted by CMC Vellore, July 2017
Ayushi Tayal was awarded Dr Ashok Chakravarti memorial award in poster competition on “ Molecular characterisation of Chikungunya virus strains isolated from 2016 Delhi outbreak revealed emergence of a novel mutation in E1 gene.” In the IAMM DC Annual conference ,Dec 2017.
Pradheer Gupta, Akshay Batra,Manish Chaudhary Guresh kumar, Meenu Bajpai,Ekta Gupta. “ Platelets as an extrahepatic reservoir of HCV-RNA : A prospective study.” Poster presentation in National virology conference, CMC Vellore , July 2017. Awarded 3rd prize.
Sujata lall, Pragya ranjan, Guresh kumar, Ekta Gupta. Quantitative IgM core antibody could be a reliable marker in differentiation of acute hepatitis B (AHB) from chronic hepatitis B with acute exacerbations (CHB-AE). Poster presentation in National virology conference, CMC Vellore , July 2017
Sujata lall, Pragya ranjan, Guresh kumar, Ekta Gupta .Poster presentation-Assessment Of Serological Assays For Solving the Dilemma Between Acute Hepatitis (AHB) And Chronic Hepatitis B with Acute Exacerbation (CHB-AE) in CIDSCON 2017 at Nagpur.
Ayushi tayal, Supriyamahajan, Priyanka jain, Ekta Gupta*. Prevalence and outcome of Herpes Virus infections in solid organ transplant recipients: A report from tertiary care transplant centre. Oral presentation at National Conference of Indian Association of Medical Microbiologists (MICROCON 2017) held at RIMS, Ranchi on 25th November 2017.
Supriya Mahajan, VijetaBajpai, Priyanka Pandey, Sonam Makhija, Ekta Gupta*. Distribution and prevalence of Hepatitis B virus genotypes in hepatitis B infected patients and their role in disease severity. Oral presentation in National conference of Indian Association of Medical Microbiologists (MICROCON 2017) held at RIMS, Ranchi on 25th November, 2017.
|Name of the course||Number of Candidates
admitted (Year 2015)
|Number of Candidates
Passed Out (Year 2015)
|PDCC course in hepato-virology||03||02|
Short Term Training
|Name of the course||Details of Candidates|
|Short Term Training in Clinical Virology||Sonam Makhija, Amity University|
|Name||Institution/strong>||Date of Visit|
|Dr. Priya abraham||CMC, Vellore||19.06.2017|
|Dr. Bharti Malhotra||SMS, Jaipur||19.06.2017|
|Dr. Nidhi Prasad||IGIMS, Patna||01.11.17 to 11.11.2017|
|Dr. Abhay||IGIMS, Patna||15.10.17|
|Dr Anita Chakravarti||MAMC,Delhi||16.12.17|
|Dr Renu Gupta||IHBAS,Delhi||16.12.17|
|Dr Manoj Kumar||Scientist, NIB|
|Dr Manjula Kiran||Scientist, NIB|