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The clinical and cellular transplantation Immunology at Institute of Liver & Biliary Sciences is a state of the art histo-compatibility and immunegenetics testing laboratory that supports busy clinical transplant programs and transplantation research activities. In ILBS overarching goal of Clinical and Cellular Transplant Immunology unit is to provide the comprehensive diagnostic tests related to liver and kidney transplants as well as for graft rejections.”
The human leukocyte antigen (HLA) test, also known as HLA typing or tissue typing, identifies antigens on the white blood cells (WBCs) that determine tissue compatibility for organ transplantation. Number of identical HLA antigens increases the probability for a successful transplant.Clinical and cellular transplantation Immunology laboratory at ILBS, we have state-of-the-art equipments to perform molecular HLA typing to identify HLA genes at low allele resolution by PCR-SSP (polymerase chain reaction-sequence-specific primers) for kidney and liver transplants.
In addition to this, laboratory also provide services for determination of HLA B-27. Human Leukocyte Antigen B*27 (subtypes B*2701-2724) is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6 and presents microbial antigens to T-cells. HLA-B27 is strongly associated with a certain set of autoimmune diseases like ulcerative colitis, inflammatory bowel disease and Crohn disease.
Patients with graft rejections may have circulating antibodies that recognize HLA antigens on a donor organ or tissue and experience rapid and irreversible destruction of the graft upon transplantation. Even low levels of antibodies may damage the grafts, so more sensitive antibody tests are used for crossmatching and for identifying the risk of immune antibody-mediated damage.Clinical and cellular transplantation Immunology laboratory at ILBS also provide services for Panel Reactive Antibody (PRA) test to rule out any reaction against pre-existing antibodies. Cross-match tests are used primarily for transplant candidates to assess the suitability of a potential donor. In renal transplant, a positive lymphocytotoxic T-cell cross-match is a contraindication for renal transplant.
Clinical and cellular transplantation Immunology laboratory performs complement-dependent cytotoxicty (CDC METHOD and HLA-Cross match by flow cytometry to detect antibodies that react with donor HLA antigens prior to transplantation
Clinical and cellular transplantation Immunology laboratory also provide services for monitoring of phenotypic characteristics of T-lymphocytes (CD4 and CD8 cells) in peripheral blood, which is commonly performed in the management of human immunodeficiency virus (HIV) positive and chronic hepatitis patients. The serial measurements of CD4 and CD8 lymphocytes to study the influence of immune suppressants are useful for a possible predictive value of lymphocyte count for the occurrence of cancer.