Biochemistry
The Department of Biochemistry makes a conscientious effort to provide the judicious and explicit use of best evidence in making decisions about the care of individual patients. Further, the department reflects an emphasis on patient care, the clinical aspects of the diseases diagnosed by the clinicians and the emerging importance of using laboratory tests to follow the progression of disease and the efficacy of therapy. The lab caters to core biochemistry, critical care, immunology and immunopathology and keeps abreast with the recent major advances in the field of clinical biochemistry. The testing methodologies span a vast panorama from colorimetry, nephelometry, CLIA, ELISA, Western Blot to IIF (microscopic diagnosis). It also plays a role in education and research, total quality management and continual quality improvement through medical , scientific, and technical expertise and optimum use of resources such as well-trained personnel, modern equipment, well-designed physical environment, data processing equipment and other supplies. Cost effective services and participation in public health initiatives for prevention of liver diseases are other hallmarks of the Biochemistry lab. Our goal is to give the right result at the right time, on the right specimen, from the right patient with right result interpretation based on correct reference data, and at the right price. These objectives represent good laboratory practice (GLP). Quality is a habit for us.
Our Laboratory activities are spread out among several units:
- Emergency Biochemistry Lab-unit: Emergency testing service is provided for 24 hours a day, 365 days a year. The emergency tests include electrolytes, enzymes, gas and pH in whole blood and body fluids. Other urgent tests such as Troponin I, Ammonia, CK, CK-MB , and NT-proBNP are also provided.
- Routine Blood Testing Unit: Tests performed in this unit include renal function, liver function, heart, specific proteins, lipid profile, iron indices,HbA1c,amylase,lipase,ACE, urine NGAL,bileacids, ELF, Fructosamine, Homocysteine, routine testing of urine and various fluids, measurement of osmolality,etc
- The Immunology Unit: This unit conducts various immunology tests and autoimmune markers by Immunofluorescence, Blot and ELISA methods for ANA, ASTHMA, p-ANCA, c-ANCA, Autoimmune Liver profile, ENA profile, ASCA,TTG, Chromogranin, PRA,S1P, Myostatin, G-CSF, Food and Inhalant Allergy and ethyl glucuronide.
- The Endocrinology and Cancer Marker Unit: Various hormonal blood tests are conducted such as Thyroid function tests, Insulin,PTH,Vit.B12,Vitamin-D3,Testosterone, Cortisol & Folate. Additionally, the unit is responsible for testing various cancer markers such as CA 125, CEA, CA 19-9, AFP, PSA, Glypican-3and PIVKA II.
- The Unit for therapeutic drug monitoring (TDM): Conducts drug level testing of the blood Tacrolimus and Cyclosporine.
- Special tests Unit: Carries out some special tests such as immunoglobulins IgG,IgA,IgM,IgE, IgG4,Total Homocysteine (indication for CAD),C3,C4,hsCRP,IL-6,TNFα.
Services Rendered by the department: From January'22 to November'23
| Name of Test | 2022 | Till Nov 2023 |
|---|---|---|
| ROUTINE | 390237 | 379161 |
| BODY FLUIDS | 14153 | 14633 |
| URINE TESTS | 6413 | 7211 |
| ENDOCRINOLOGY | 35766 | 40176 |
| ONCOLOGY | 16320 | 19708 |
| IMMUNOLOGY | 5922 | 2555 |
| INFLAMMATORY MARKERS | 13071 | 16019 |
| SEROLOGY | 3834 | 1669 |
| AUTOIMMUNE MARKERS | 3704 | 3740 |
| THERAPEUTIC DRUG MONITORING | 6605 | 5902 |
| LIVER TRANSPLANT CHEMISTRY | 137 | 166 |
| BONE METABOLISM | 5163 | 6162 |
| POCT | 183282 | 169768 |
| ALLERGY PROFILE | 181 | 198 |
| OUT SOURCED TESTS | 2068 | 2266 |
| NEW TESTS | 462 | 1101 |
| TOTAL TESTS | 687318 | 670435 |
| New services & facilities added to the department in 2023: | |
|---|---|
| • Enhanced Liver Fibrosis Panel, | • Glypican-3 |
| • Fructosamine | • Adiponectin |
| • Cyclosporine | • IL-10 |
| • Non-esterified fatty acid NEFA | • Autoimmune liver profile |
| • Ketone bodies | • Automated urine NGAL |
| • Both human and rat serum NF-kB- P65 | • EGFR |
| • IL-10 | • FIB-4 score |
| • Rat Adiponectin | • APRI score |
| • Hepatocyte growth factor(HGF), | • Apo-A |
| • Stromal derived factor(SDF-1), | • Apo-B |
| • Vascular endothelial growth factor(VEGF), | • Lp(a) |
| • Granulocyte stimulating factor(G-CSF) | • Angiopoetin-2 |
| • Insulin like growth factor(IGF), | • Inhalant allergy profile |
| • NAD/NADH, | • Apoprotein profile |
| • S1P | • Extended lipid profile |
| • Myostatin | • Glucose Challenge Test |
New Facility - NA
New instrument
- Atellica immunoassay analyzer
- Osmometer
Educational activities:
- Details of the Educational activities (CME/Conf/workshop) hosted by the department in 2023:
- Attended ANA masterclass(CME and Workshop on Autoimmune Diagnostics in April 2023 sponsored by Medsource
- Attended Quality Control Symposium organized by Bio-Rad Laboratories on 12.6.23
- Was Chairperson for Guest Lecture by Professor W.Ray Kim on the topic post-LT immunosuppressionon 4.6.23 in the DRILLS Conference(Decisions Reasoning Innovations Learning in Liver Disease) at Hotel Andaz, Aerocity. New Delhi
- Took a class for Nurses at ILBS on Overview of Biochemistry Lab: An interactive session for gap closure on 21.6.23.
- Moderator for seminars of DM Hepatology students 'classes at ILBS.
- Abstract titled- “Diagnostic assessment of PIVKA-II in the detection of hepatocellular carcinoma in Indian patients”was selected for APASL 2024.
Courses offered by the Department
| Course |
|---|
| PDCC in Advanced Clinincal Biochemistry |
Department's Achievements: Department' research activity as well as residents achievments:
- Mylan Project-“Healthy Liver Healthy Delhi”
- Judge of posters and videos for 26th Hepatitis Day on 4.12.2023
- Successfully participated in NABL, NABH & KAYAKALP audit
| Visiting faculty to the department in 2023: |
|---|
| Dr. Ritu Singh Professor & Head, Lady Hardinge Medical College, New Delhi |
