Speciality

Research

Dr Gayatri Ramakrishna  
Dr Gayatri Ramakrishna
Additional Professor
 
Experience

Research Experience:

  • Research associate, All India Institute of Medical Sciences, New Delhi, India
  • Visiting Fogarty Fellow, NIH, Laboratory of Comparative Carcinogenesis, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland, USA
  • Staff Scientist V, Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, India  (till Sept 2012).           
  • Associate Professor, Dept of Research, Institute of Liver and Biliary Sciences (ILBS), Delhi
Qualification

Dr. Gayatri graduated from Jawaharlal Nehru University and perused her postdoctoral work at National Cancer Institute, NIH, USA. Prior to joining ILBS she work as staff scientist at CDFD, Hyderabad. Her areas of research interests include understanding mechanism, which are important in stress response. Her current work involves identifying novel hepatostats i.e growth  inhibitors leading to  senescence or quiescence

Number of publications
52
Research Int

The laboratory of cancer cell biology focuses on the following areas:

  • Role of signaling pathways during neoplastic transformation: Our group had previously proposed a role of wild type Ras in growth arrest and in continuation with this we made an unusual observation on significantly low amounts of wild type Ras-p21 in hepatocellular carcinoma (Bose et al., 2011). These results have prompted revaluation on newer role of wild type Ras as a possible growth suppressor and a stochastic model for progression of hepatic cancer. 
  • Epigenetic modulations by histone deacetylases during carcinogenesis and senescence:  Currently trying to understand the role of NAD+ dependent deacetylases namely Sirtuins. Amongst all the various Sirtuin members, SIRT7 is the least explored in context of cellular survival and neoplasia. Our work has thrown light on its subcellular localization and role in cellular survival ( Kiran et al, 2013).  Since cellular senescence which is at crossroads of both ageing and cancer we are trying to understand the impact of stress induced premature senescence in context of liver disease progression (Ramakrishna, 2012).
    • Immunophillins as new targets for cancer therapeutic.  In search for newer drug targets in cancer therapeutics, work from our group is suggestive of a caspase independent cell death in cancer cells following inhibition of cyclophilins (Babul  et al, 2014).
Publications
  1. Ramakrishna G, Rastogi A, Trehanpati N, Sen B, Khosla R, Sarin S.K. From Cirrhosis to Hepatocellular Carcinoma: New Molecular Insights on Inflammation and Cellular Senescence. (2013) Liver Cancer 2:367–383
  2. Kiran S, Chatterjee N, Singh S, Kaul SC, Wadhwa R, Ramakrishna G. Intracellular  distribution of human SIRT7 and mapping of the nuclear/nucleolar localization signal. (2013) FEBS J. 280(14):3451-66.
  3. Ramakrishna G, Anwar T, Angara RK, Chatterjee N, Kiran S, Singh S.  ( 2012) Role of cellular senescence in hepatic wound healing and carcinogenesis. Eur J Cell Biol. Oct;91(10):739-47. (on the cover page of the journal)
  4. Chatterjee N, Kiran S, Islam N, Ramasarma T, Ramakrishna G. (2011) Diperoxovanadate can substitute for H(2)O(2) at much lower concentration in inducing features of premature cellular senescence in mouse fibroblasts (NIH3T3). Mech Ageing Dev. 2011 May;132(5):230-9.
  5. Bose S, Sakhuja P, Bezawada L, Agarwal AK, Kazim SN, Khan LA, Sarin SK, Ramakrishna G. Hepatocellular carcinoma with persistent hepatitis B virus infection shows unusual downregulation of Ras expression and differential response to Ras mediated signaling. J Gastroenterol Hepatol. 2011 Jan;26(1):135-44.
  6. Piyathilake CJ, Badiga S, Paul P, Vijayaraghavan K, Vedantham H, Sudula M, Sowjanya P, Ramakrishna G, Shah KV, Partridge EE, Gravitt PE. Indian women with higher serum concentrations of folate and vitamin B12 are significantly less likely to be infected with carcinogenic or high-risk (HR) types of human papillomaviruses (HPVs). Int J Womens Health. 2010 Aug 9;2:7-12
  7. Gravitt PE, Paul P, Katki HA, Vendantham H, Ramakrishna G, Sudula M, Kalpana B, Ronnett BM, Vijayaraghavan K, Shah KV; CATCH Study Team.Effectiveness of VIA, Pap, and HPV DNA testing in a cervical cancer screening program in a peri-urban community in Andhra Pradesh, India. PLoS One. 2010 Oct 28;5(10):e13711.
  8. Vedantham H, Silver MI, Kalpana B, Rekha C, Karuna BP, Vidyadhari K, Mrudula S, Ronnett BM, Vijayaraghavan K, Ramakrishna G, Sowjanya P, Laxmi S, Shah KV, Gravitt PE; CATCH Study Team. Determinants of VIA (Visual Inspection of the Cervix After Acetic Acid Application) positivity in cervical cancer screening of women in a peri-urban area in Andhra Pradesh, India. Cancer Epidemiol Biomarkers Prev. 2010 May;19(5):1373-80.
  9. Sowjanya AP, Paul P, Vedantham H, Ramakrishna G, Vidyadhari D, Vijayaraghavan K, Laksmi S, Sudula M, Ronnett BM, Das M, Shah KV, Gravitt PE; Community Access to Cervical Health Study Group.  Suitability of self-collected vaginal samples for cervical cancer screening in periurban villages in Andhra Pradesh, India. Cancer Epidemiol Biomarkers Prev. 2009 May;18(5):1373-8.
  10. Gokul G, Ramakrishna G, Khosla S. Reprogramming of HeLa cells upon DNMT3L overexpression mimics carcinogenesis. Epigenetics. 2009 Jul;4(5):322-9.
  11. Gokul G, Gautami B,Malathi S, Sowjanya AP, Poli UR, Jain M,  Ramakrishna G,Khosla S. (2007)   DNA methylation profile at the DNMT3L promoter: a potential biomarker for cervical cancer. Epigenetics. 2007 2(2):80-5.
  12. Sowjanya AP, Jain M, Poli UR, Padma S, Das M, Shah KV, Rao BN, Devi RR, Gravitt PE, Ramakrishna G. (2005) Prevalence and distribution of high-risk Human papilloma virus (HPV) types in invasive squamous cell carcinoma of the cervix and in normal women in Andhra Pradesh, India. BMC Infect Dis.;5(1):116  (Highly accessed paper)
  13. Padma S, Sowjanya AP, Poli UR, Jain M, Rao B, Ramakrishna G. S. Padma , A. Pavani Sowjanya, Usha Rani Poli, Meenakshi Jain, B. N. Rao  and Ramakrishna G. (2005) Downregulation of Calcineurin Activity in Cervical Carcinoma. Cancer Cell International , 5:7
  14. Singh A, Sowjanya AP, Ramakrishna G. Arvind Singh, Pavani Sowjanya and Ramakrishna G. (2005) The Wild type Ras: Road ahead. FASEB Journal  19 : 161-9.
  15. Vucenik I, Ramakrishna G, Tantivejkul K, Anderson LM, Ramljak D.  (2005) Inositol hexaphosphate (IP6) blocks proliferation of human breast cancer cells3 through a PKCd-dependent increase in p27Kip1 and decrease in retinoblastoma protein (pRb) phosphorylation. Breast Cancer Res Treat. 91(1):35-45.
  16. Radha Rama Devi, Govindiah V, Ramakrisna G and Naushad M  (2004) Prevalance of methylene tertahydrofolate reducatse polymorphism in south Indian population. Current Science  86, 440-3
  17. Kammouni W*, Ramakrishna G*, Sithanandam G, Smith GT, Fornwald LW, Masuda A, Takahashi T, Anderson LM. (2002) Increased K-ras protein and activity in mouse and human lung epithelial cells at confluence. Cell Growth Differ.,13(9):441-8. (* equal contribution by both authors)
  18. Calvert RJ, Ramakrishna G, Tepper S, Diwan BA, Anderson LM,  Kritchevsky D. (2002) Alterations in membrane-bound and cytoplasmic K-ras protein levels in mouse lung induced  by treatment with lovastatin, cholestyramine, or niacin: effects are highly mouse strain  dependent. Biochem Pharmacol. 1;64(1):41-8.
  19. Ramakrishna G, Perella C, Birely L, Diwan BA, Fornwald LW, Anderson LM. (2002) Decrease in K-ras p21 and increase in Raf1 and activated Erk 1 and 2 in murine lung tumors initiated by N-nitrosodimethylamine and promoted by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Appl Pharmacol. 15;179(1):21-34.
  20. Shiao YH, Ramakrishna G, Anderson LM, Perantoni AO, Rice JM, Diwan BA. (2002) Down-regulation of     von Hippel-Lindau protein in N-nitroso compound-induced rat non-clear cell renal tumors  Cancer Lett., 8;179(1):33-8.
  21. McKenna IM, Ramakrishna G, Diwan BA, Kang Y, Shiao YH, Wakefield LM, Powell DA, Anderson LM, Jakowlew SB. (2001) Heterozygous inactivation of TGF-beta1 increases the susceptibility to  chemically induced mouse lung tumorigenesis independently of mutational activation of K-ras. Toxicol Lett., 15;123(2-3):151-8.
    22. McKenna IM, Ramakrishna G, Diwan BA, Shiao YH, Kasprzak KS, Powell DA, Anderson LM. (2001) K-ras mutations in mouse lung tumors of extreme age: independent of paternal preconceptional exposure to chromium(III) but significantly more frequent in carcinomas than adenomas. Mutat Res., 25;490(1):57-65.
  22. Ramakrishna G, Sithanandam G, Cheng RY, Fornwald LW, Smith GT, Diwan BA, Anderson LM. (2000) K-ras p21 expression and activity in lung and lung tumors. Exp Lung Res., 26(8):659-71.
  23. Ramakrishna G, Bialkowska A, Perella C, Birely L, Fornwald LW, Diwan BA, Shiao YH, Anderson   LM. (2000) Ki-ras and the characteristics of mouse lung tumors. Mol Carcinog. 28(3):156-67.
  24. Diwan BA, Ramakrishna G, Anderson LM, Ramljak D. (2000)  Overexpression of Grb2 in inflammatory   lesions and preneoplastic foci and tumors induced by N-  nitrosodimethylamine in Helicobacter  hepaticus-infected and -noninfected A/J mice. Toxicol Pathol. 28(4):548-54.
  25. Shiao YH, Resau JH, Nagashima K, Anderson LM, Ramakrishna G. The von Hippel-Lindau tumor suppressor targets to mitochondria. Cancer Res. 2000, 1;60(11):2816-9.
  26. Rice PL, Porter SE, Koski KM, Ramakrishna G, Chen A, Schrump D, Kazlauskas A, Malkinson  AM. (1999) Reduced receptor expression for platelet-derived growth factor and epidermal growth factor in dividing mouse lung epithelial cells. Mol Carcinog. 25(4):285-94.
  27. Sithanandam G, Ramakrishna G, Diwan BA, Anderson LM.  (1998) Selective mutation of K-ras by N-ethylnitrosourea shifts from codon 12 to codon 61 during fetal mouse lung maturation. Oncogene. 30;17(4):493-502.
  28. Ramakrishna G,       Anderson LM. (1998) Levels and membrane localization of the c-K-ras p21 protein in lungs of mice of different genetic strains and effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Aroclor 1254. Carcinogenesis. 19(3):463-70.
  29. Ramakrishna G ,  Chatterjee S. (1994) Morphogenesis of Dictyostelium discoideum treated with lindane. Bull Environ Contam Toxicol. 52(6):871-7.
  30. Ramakrishna G , Chatterjee S (1994) Pinocytic stimulation in Dictyostelium discoideum by gamma-benzene hexachloride. J Cell Physiol. 158(3):523-6.
  31. Ramakrishna G ,  Chatterjee S  (1994) Growth and development of cellular slime mould Dictyostelium discoideum treated with DDT. Environ Pollut.;86(2):135-40.
  32. Ramakrishna G , Chatterjee S.  (1993) Phosphatase activities in pesticide-treated growing and developing cells of Dictyostelium discoideum. J Appl Toxicol. Jul-Aug;13(4):297-300.
  33. Ramakrishna G,  Chatterjee S. (1993) Phagocytic activity of Dictyostelium amoebae treated with an organochlorine pesticide. Cell Biol Int. 17(3):349-52.
  34. Ramakrishna G , Chatterjee S. (1992) Effects of lindane on growth of cellular slime mold Dictyostelium discoideum. Bull Environ Contam Toxicol. Aug;49(2):285-9.
  35. Ramakrishna G , Chatterjee S. (1991) Effects of chlorpromazine on growth and development of Dictyostelium discoideum. Microbios.;68(275):97-107.
  36. Ram BM, Ramakrishna G. Endoplasmic reticulum vacuolation and unfolded protein response leading to paraptosis like cell death in cyclosporine A treated cancer cervix cells is mediated by cyclophilin B inhibition. (2014)   BBA: Molecular Cell Research  (In Press)
  37. Singh S, Kumar U, Thakur S, Kran S, Sen B, Sharma S, Venkaiah V, Poongothai AR and Ramakrishna G (2015)  Expression/localization patterns of Sirtuins (SIRT1, SIRT2 and SIRT7) during progression of cervical cancer and effects of Sirtuin inhibitors on growth of cervical cancer cells   Tumor Biology (in press)
  38. Kiran S, Oddi V, Ramakrishna G. (2015)   Sirtuin 7 promotes cellular survival following genomic stress by attenuation of DNA damage, SAPK activation and p53 response. Exp Cell Res.  Feb 1;331(1):123-41.
  39. Kiran S, Anwar T, Kiran M, Ramakrishna G (2015) Sirtuin 7 in cell proliferation, stress and disease: Rise of the Seventh Sirtuin! Cell Signal 27(3):673-682
  40. Ram BM, Ramakrishna G. (2014)  Endoplasmic reticulum vacuolation and unfolded protein response leading to paraptosis like cell death in cyclosporine A treated cancer cervix cells is mediated by cyclophilin B inhibition. Biochim Biophys Acta. 1843(11):2497-512.

Submission to NCBI : Single nucleotide polymorphism  database:
Maryam Bashir, A.P.Suresh Kumar,  Ramakrishna G   (2004) A synonymous SNP in codon 1493 of adenomatous polyposis coli gene in Indian population.

  • Total Number of publications of that faculty member:                                             52
  • Total Number of publications in 2018:                                                                       3               
  • Total Number of projects 2018                                                                                    2

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